你所不知道的引起武汉新型冠状病毒肺炎的病毒

发表时间：2020-01-31 08:33

截至今天一月三十一日早上，美国国立卫生研究院病毒数据库有六株武汉新型肺炎冠状病毒全长测序，大陆两个，香港两个，美国CDC两个，序列与中国测序结果全部一致。也就是说，目前在中国传播并扩散到诸多国家，来源是同一病毒。

检索美国NIH病毒数据库，一共有56株萨斯病毒，全部由华人测得，与武汉新型肺炎冠状病毒同源性高，全部都在80%以上。可以认为，它就是SARS的变种。

138株蝙蝠冠状病毒，分布于全世界二十多个国家，与武汉新型肺炎冠状病毒同源性非常高。最高一株为南京军事医学科研所，同源性达89.12%，还有一株也来自南京军事医学研究所，同源性为88.65%。基本可以认为，蝙蝠冠状病毒的变异，生成了武汉新型肺炎冠状病毒。

573株中东冠状病毒，分布在世界各地。与武汉肺炎病毒同源性低，在70%左右。基本可以排除这个病毒引起武汉新型冠状病毒肺炎的可能性。

879株猪冠状病毒，分布世界各地。与武汉肺炎病毒同源性低，在60-65%左右。

以下就以MN908947.3以为例，进行全基因组比较，找出它的同源性，Wuhan seafood market pneumonia virus isolate Wuhan-Hu-1, （2020-01-23）GenBank: MN908947.3

第一部分：与SARS冠状病毒比较

SARS coronavirus GD01,（军事医学科学院，2003）

GenBank: AY278489.2

Percentage of Identity: 82.24%

SARS coronavirus BJ02, （军事医学科学院，2003）

GenBank: AY278487.3

Percentage of Identity: 82.25%

SARS coronavirus BJ01, （军事医学科学院，2003）

GenBank: AY278488.2

Percentage of Identity: 82.01%

第二部分：蝙蝠SARS冠状病毒比较

Bat SARS-like coronavirus isolate bat-SL-CoVZXC21, （南京军事医学研究所，2018）

GenBank: MG772934.1

Percentage of Identity: 88.65%

Bat SARS-like coronavirus isolate bat-SL-CoVZC45, （南京军事医学研究所 2018 ）

GenBank: MG772933.1

Percentage of Identity: 89.12%

Bat coronavirus BtCoV/Rh/YN2012 isolate BtCoV/Rh/YN2012_Rs4259 （武汉病毒所 2019）

GenBank: MG916903.1

Percentage of Identity: 63.69%

Bat SARS CoV Rs672/2006, （武汉病毒所）2010

GenBank: FJ588686.1

Percentage of Identity: 83.25%

Bat coronavirus Vs-CoV-1 genomic RNA, nearly complete genome （日本，2019）

GenBank: LC469308.1

Percentage of Identity: 72.91%

Bat coronavirus strain BtCoV92, complete genome （新加坡2019）

GenBank: MK492263.1

Percentage of Identity: 71.37%

Bat coronavirus CDPHE15/USA/2006, complete genome（NIAID, 2018）

NCBI Reference Sequence: NC_022103.1

Percentage of Identity: 80.91%

Bat coronavirus BM48-31/BGR/2008, complete genome （德国，2018）

NCBI Reference Sequence: NC_014470.1

Percentage of Identity: 80.44%

Zaria bat coronavirus strain ZBCoV, partial genome （尼日利亚，2016）

GenBank: HQ166910.1

Percentage of Identity: 71.12%

Bat coronavirus CDPHE15/USA/2006, complete genome （美国NIAID）

GenBank: KF430219.1

Percentage of Identity: 80.91%

Bat coronavirus BM48-31/BGR/2008, complete genome （德国，2010）

GenBank: GU190215.1

Percentage of Identity: 80.44%

第三部分：与中东冠状病毒比较（mers CoV）

Middle East respiratory syndrome-related coronavirus isolate MERS-CoV camel/Kenya/C1272/2018, complete genome （肯尼亚，2019）

GenBank: MH734115.1

Percentage of Identity: 71.31%

Middle East respiratory syndrome-related coronavirus isolate MERS-CoV camel/Nigeria/NV1673/2016, complete genome （尼日利亚，2016）

GenBank: MG923478.1

Percentage of Identity: 71.15%

Middle East respiratory syndrome coronavirus strain ChinaGD01, complete genome （中国 2016）

GenBank: KT006149.2

Percentage of Identity: 71.15%

Middle East respiratory syndrome coronavirus isolate Qatar4, complete genome（英国，2014）

GenBank: KF961222.1

Percentage of Identity: 71.15%

Middle East respiratory syndrome coronavirus isolate KSA-CAMEL-378, complete genome（沙特，2014）

GenBank: KJ713296.1

Percentage of Identity: 71.07%

第四部分：全部蝙蝠冠状病毒基因登记号

1. Bat SARS coronavirus Rf1,

29,709 bp Accession: DQ412042.1 GI: 89514809

2. Bat SARS coronavirus Rm1,

29,749 bp Accession: DQ412043.1 GI: 89514824

3.Bat SARS CoV Rs672/2006,

29,059 bp Accession: FJ588686.1 GI: 255733149

4.Bat SARS coronavirus Rp3,

29,736 bp Accession: DQ071615.1 GI: 72256267

5.Bat SARS coronavirus HKU3-1,

29,728 bp Accession: DQ022305.2 GI: 76160337

6.bat SARS coronavirus HKU3-3,

29,711 bp Accession: DQ084200.1 GI: 71796716

7.bat SARS coronavirus HKU3-2,

29,687 bp Accession: DQ084199.1 GI: 71796704

8. Bat SARS coronavirus HKU3-13,

29,677 bp Accession: GQ153548.1 GI: 292660245

9.Bat SARS coronavirus HKU3-12,

29,704 bp Accession: GQ153547.1 GI: 292660233

10.Bat SARS coronavirus HKU3-11,

29,695 bp Accession: GQ153546.1 GI: 292660221

11.Bat SARS coronavirus HKU3-10,

29,695 bp Accession: GQ153545.1 GI: 292660209

12.Bat SARS coronavirus HKU3-9,

29,695 bp Accession: GQ153544.1 GI: 292660197

13.Bat SARS coronavirus HKU3-8,

29,681 bp Accession: GQ153543.1 GI: 292660183

14.Bat SARS coronavirus HKU3-7,

29,716 bp Accession: GQ153542.1 GI: 292660171

15.Bat SARS coronavirus HKU3-6,

29,704 bp Accession: GQ153541.1 GI: 292660159

16.Bat SARS coronavirus HKU3-5,

29,704 bp Accession: GQ153540.1 GI: 292660147

17.Bat SARS coronavirus HKU3-4,

29,704 bp Accession: GQ153539.1 GI: 292660135

18.Bat SARS-like coronavirus isolate bat-SL-CoVZXC21,

29,732 bp Accession: MG772934.1 GI: 1369125429

19.Bat SARS-like coronavirus isolate bat-SL-CoVZC45,

29,802 bp Accession: MG772933.1 GI: 1369125417

20.Bat SARS-like coronavirus Rs3367,

29,792 bp Accession: KC881006.1 GI: 556015127

21.Bat SARS-like coronavirus RsSHC014,

29,787 bp Accession: KC881005.1 GI: 556015114

22.Bat SARS-like coronavirus WIV1,

30,309 bp Accession: KF367457.1 GI: 556015142

23. Bat SARS-like coronavirus isolate Rs9401,

29,769 bp Accession: KY417152.1 GI: 1270541521

24.Bat SARS-like coronavirus isolate Rs7327,

30,307 bp Accession: KY417151.1 GI: 1270541507

25.Bat SARS-like coronavirus isolate Rs4874,

30,311 bp Accession: KY417150.1 GI: 1270541493

26.Bat SARS-like coronavirus isolate Rs4255,

29,743 bp Accession: KY417149.1 GI: 1270541480

27.Bat SARS-like coronavirus isolate Rs4247,

29,743 bp Accession: KY417148.1 GI: 1270541467

28.Bat SARS-like coronavirus isolate Rs4237,

29,741 bp Accession: KY417147.1 GI: 1270541454

29.Bat SARS-like coronavirus isolate Rs4231,

29,782 bp Accession: KY417146.1 GI: 1270541441

30.Bat SARS-like coronavirus isolate Rf4092,

29,710 bp Accession: KY417145.1 GI: 1270541428

31.Bat SARS-like coronavirus isolate Rs4084,

29,770 bp Accession: KY417144.1 GI: 1270541414

32.Bat SARS-like coronavirus isolate Rs4081,

29,741 bp Accession: KY417143.1 GI: 1270541401

33.Bat SARS-like coronavirus isolate As6526,

29,725 bp Accession: KY417142.1 GI: 1270541388

34.Bat coronavirus Cp/Yunnan2011,

29,452 bp Accession: JX993988.1 GI: 442796484

35.Bat coronavirus Rp/Shaanxi2011,

29,484 bp Accession: JX993987.1 GI: 442796476

36.Bat SARS-like coronavirus YNLF_34C,

29,723 bp Accession: KP886809.1 GI: 916354756

37.Bat SARS-like coronavirus YNLF_31C,

29,723 bp Accession: KP886808.1 GI: 916354744

38.Bat coronavirus (BtCoV/279/2005),

29,741 bp Accession: DQ648857.1 GI: 109893933

39.Bat coronavirus (BtCoV/273/2005),

29,704 bp Accession: DQ648856.1 GI: 109893923

40.SARS-related bat coronavirus isolate Longquan-140 orf1ab polyprotein, spike glycoprotein, envelope protein, membrane protein, and nucleocapsid protein genes, complete cds

29,676 bp Accession: KF294457.1 GI: 656462529

41.SARS-related bat coronavirus isolate Anlong-111 orf1ab polyprotein and orf1a polyprotein genes, complete cds

21,479 bp Accession: KF294455.1 GI: 656462524

42.SARS-related bat coronavirus isolate Jiyuan-331 orf1ab polyprotein gene, complete cds

21,444 bp Accession: KF294456.1 GI: 656462527

43.BtRf-BetaCoV/JL2012,

29,037 bp Accession: KJ473811.1 GI: 641457765

44.Bat coronavirus BtCoV/Rh/YN2012 isolate BtCoV/Rh/YN2012_Rs4259,

29,076 bp Accession: MG916903.1 GI: 1597473558

45.Bat coronavirus BtCoV/Rh/YN2012 isolate BtCoV/Rh/YN2012_Rs4125,

29,074 bp Accession: MG916902.1 GI: 1597473545

46.Bat coronavirus BtCoV/Rh/YN2012 isolate BtCoV/Rh/YN2012_Rs3376,

29,102 bp Accession: MG916901.1 GI: 1597473532

47. Bat coronavirus BM48-31/BGR/2008,

29,276 bp Accession: NC_014470.1 GI: 304633675

48. BtRs-BetaCoV/HuB2013,

29,658 bp Accession: KJ473814.1 GI: 641457801

49. BtRf-BetaCoV/SX2013,

29,461 bp Accession: KJ473813.1 GI: 641457790

50.BtRf-BetaCoV/HeB2013,

29,443 bp Accession: KJ473812.1 GI: 641457779

51. UNVERIFIED: SARS-related coronavirus isolate F46,

29,722 bp Accession: KU973692.1 GI: 1189483555

52. BtRs-BetaCoV/YN2013,

29,142 bp Accession: KJ473816.1 GI: 641457823

53. BtRs-BetaCoV/GX2013,

29,161 bp Accession: KJ473815.1 GI: 641457812

54.Bat coronavirus BM48-31/BGR/2008,

29,276 bp Accession: GU190215.1 GI: 301298998

55.Bat coronavirus strain 16BO133,

29,075 bp Accession: KY938558.1 GI: 1220516375

56.SARS-like coronavirus WIV16,

30,290 bp Accession: KT444582.1 GI: 940378824